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KMID : 0381120110330020155
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Genes and Genomics
2011 Volume.33 No. 2 p.155 ~ p.161
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Molecular cloning of novel α-gliadin genes from Crithopsis delileana and the evolution analysis with those from Triticeae
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Guo Zhi-Fu
Long Xiang-Yu
Dong Pan
Wei Yu-Ming
Bai Li-Ping
Dang Xiao-Xuan
Wan Hao-Lei
Zhang Li-Jun
Zheng You-Liang
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Abstract
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The α-gliadins from Crithopsis delileana (Schult) Roshev (2n=2x=14, KK) were investigated by Acid polyacrylamide gel electrophoresis (A-PAGE) analysis. It was indicated that the electrophoresis mobility of gliadins from C.delileana had obvious difference with those from common wheat in α, γ and ω region. Using primers designed from published sequences of α-gliadin genes, three α-gliadin genes were isolated from C. delileana, which were designated as gli-ka1, gli-ka2 and gli-ka3, respectively. Two in-frame stop codons were found in the coding sequences of gli-ka3, indicating that gli-ka3 could be a pseudogene. The gli-ka2 was a gliadin with an odd number of cysteines, resulting from a non-synonymous mutation. This change might lead to the interactive behavior of gli-ka2. Three α-gliadin genes of C. delileana had the similar but not identical primary structures to the corresponding gene sequences from other wheat related species. By the alignment of α-gliadin genes from Triticeae, phylogenetic analysis indicated that three α-gliadin genes of C. delileana clustered together with all α-gliadin genes from Ee genome of Lophopyrum elongatum by an interior paralleled branch.
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KEYWORD
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Crithopsis delileana, α-gliadin gene, Phylogenetic analysis, Triticeae
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